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KMID : 0380020040190040327
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 4 p.327 ~ p.330
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Production of Enantiomerically Pure [R]-3-Hydroxybutyric acid by Metabolically Engineered Escherichia coli with Inducible System
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Lee Young
Choi Jong-Il
Lee Sang-Yup
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Abstract
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An inducible expression system of poly[(R)-3-hydroxybutyrate] (PHB) depolymerization was established in metabolically engineered Escherichia coli with the PHB biosynthesis genes. The Ralstonia eutropha PHB depolymerase gene was cloned in a vector system containing the PHB biosynthesis genes and expressed under inducible promoter. Recombinant E. coli harboring the PHB biosynthesis genes and depolymerase gene was first cultured for the accumulation of PHB, and then the depolymerase was expressed resulting in the degradation of accumulated PHB into (R)-3-hydroxybutyric acid (R3HB). R3HB could be produced with the concentration of 7.6 g/L in flask culture. Two different PHB biosynthesis genes from Alcaligenes latus and R. eutropha were compared for the production of R3HB. This strategy can be used for the production of enantiomerically pure (R)-hydroxycarboxylic acids with high concentration.
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KEYWORD
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(R)-3-Hydroxybutyric acid, inducible depolymerization, recombinant E. coli, PHB
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